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Biacore™ analysis

Agro-Bio can analyse your antibodies’ interaction capabilities on its Biacore™ system, for optimal characterization based on your needs and applications.

Biacore™ analysis of your antibodies’ molecular interactions

Agro-Bio’s Biacore™ T200 is a highly sensitive system for real-time biomolecular interaction analysis, based on surface plasmon resonance (SPR) technology. The molecule of interest is immobilized on a sensor chip, then a solution of a potential interaction partner is injected onto the chip. The interaction between the molecules is then observed in real time via the optical system.

This instrument enables us to perform various interaction analyses on your antibodies, in order to:

  • Screen hybridoma supernatants to select which hybridomas to clone
  • Determine an antibody’s affinity constants for its target antigen: dissociation equilibrium constant KD, association rate constant ka (kon) and dissociation rate constant kd (koff)
  • Study the binding of an IgG antibody to FcγR receptors: antibody-dependent cellular cytotoxicity (ADCC), etc.
  • Study the binding of an IgG antibody to the FcRn (neonatal) receptor: IgG recycling (the antibodies’ half-life in the blood).

We adapt and optimize the assay format based on your antibody and the analysis requested.

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