Agro-Bio offers several kinds of purification for biological products with easy and fast methods to be set up and not to denature products.
Purification by precipitation is an alternative to the protein A/G purification by performing the purification of total antibodies from serum, in vitro supernatant or ascites by precipitation with caprylic acid and with ammonium sulfate.
Purification ion exchange is based on the principle of hydrophobic charge-induction chromatography. It engages hydrophobic interactions with the immunoglobulins or other proteins, when they are positively or negatively charged. This technique is mainly used to purify specific IgM (negatively charged).
Size exclusion chromatography is a method for separating molecules based on the filtration of different sizes of molecules through a porous gel.
Proteins in solution, or other macromolecules, are applied to a column with a defined support medium. The behaviour of the protein depends on its size and that of the pores in the medium. Thus the different proteins are eluted in the reverse order of their molecular weight.