Epitope mapping is a method for identifying antibody-binding sites on an antigen.
This benefit combines:
- proteolytic digestion steps,
- an isolation step of the epitope peptides by affinity chromatography,
- the final analysis of the epitope fragments by mass spectrometry.
- The Antigen/Antibody complex is formed in the affinity chromatography and the digestion of the complex is performed in the affinity column,
- The digestion of the antigen is performed before the formation of Antigen/Antibody in the affinity column.
The search for this sequence of interest, proposed by Agro-Bio, is based on the protection of the epitope against enzymatic digestion by the formation of Antigen/Antibody complex.
After affinity chromatography and digestion, the fragments of the epitope are eluted and then sequenced by mass spectrometry (MALDI TOF-TOF).