The 1D gel electrophoresis are made on polyacrylamide gel. To view the results and according to the desired sensitivity threshold, gels can be stained with:
- sensitivity 4 to 8 ng protein
- compatible with quantifications of studies and mass spectrometry
- sensibility 0.5 to 1 ng protein
- compatible with the mass spectrometry or by any other method depending on the need.
Western blot (immunoblot also semi-dried) is a technique for the transfer on nitrocellulose of separated proteins by electrophoresis. It allows to detect and to identify specific proteins in a biological sample or to test antibodies led against these proteins.