ServicesDevelopment and production of antibodies > Polyclonal antibodies development

Polyclonal antibodies development

Immunization protocols

Agro-Bio has developed a time-optimized immunization protocol, yielding quality antibodies, needing low amounts of antigen. We recommend the use of several animals in parallel in order to overcome the differences obtained due to the genetic variability between each animal.

Short protocols 

Animal species

Number of animals by standard protocol

Duration of the standard protocol in days

Total quantity obtained

Rabbit

2

42

From 60 to 196 mL of antiserum

Mouse

5

35

From 250 to 500 µL of antiserum

Rat

3

35

From 1.5 to 2.1 mL of antiserum

Chicken

2

35

From 36 to 50 mL of IgY pre-purified in a concentration > 4 mg/mL

Long protocols

Animal species

Number of animals by standard protocol

Duration of the standard protocol in days

Total quantity obtained

Rabbit

2

63

From 60 to 72 mL of antiserum

Rabbit

2

77

From 90 to 108 mL of antiserum

Chicken

2

70

≈ 80 mL of IgY pre-purified in a concentration > 4 mg/mL

Needs of antigen for standard protocol

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